2FNJ

Crystal structure of a B30.2/SPRY domain-containing protein GUSTAVUS in complex with Elongin B and Elongin C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	128
Detector technology	CCD
Collection date	2005-04-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0064
Spacegroup name	P 21 21 21
Unit cell lengths	50.308, 82.489, 118.966
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
Rwork	0.218
R-free	0.24720
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	1.312
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	RESOLVE (2.08)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.069	0.351
Number of reflections	44051	3736
<I/σ(I)>	16
Completeness [%]	94.0	94
Redundancy	4.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	295	0.2M di-Ammonium Tartrate, 20% w/v Polyethylene Glycol 3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K
1	VAPOR DIFFUSION, HANGING DROP	6.6	295	0.2M di-Ammonium Tartrate, 20% w/v Polyethylene Glycol 3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K