2FMY
CO-dependent transcription factor CooA from Carboxydothermus hydrogenoformans (Imidazole-bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.654, 93.930, 91.901 |
| Unit cell angles | 90.00, 104.73, 90.00 |
Refinement procedure
| Resolution | 32.330 - 2.200 |
| R-factor | 0.2092 |
| Rwork | 0.207 |
| R-free | 0.24732 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.365 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 51245 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 280 | 100mM Bis-Tris buffer, 30% polyethyleneglycol 3000, 5% Dioxane, 4.5% Glycerol, pH 6.0, VAPOR DIFFUSION, temperature 280K |
