2FMP
DNA Polymerase beta with a terminated gapped DNA substrate and ddCTP with sodium in the catalytic site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-24 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.900, 80.400, 55.300 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 17.180 - 1.650 |
| R-factor | 0.179 |
| Rwork | 0.179 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bpy |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.041 | 0.249 |
| Number of reflections | 49071 | |
| <I/σ(I)> | 4.25 | |
| Completeness [%] | 96.0 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM HEPES, pH 7.5, 180 mM Sodium Acetate, 16 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | HEPES | ||
| 2 | 1 | 1 | Sodium Acetate | ||
| 3 | 1 | 1 | PEG 3350 | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium Acetate | ||
| 6 | 1 | 2 | PEG 3350 | ||
| 7 | 1 | 2 | H2O |
