2FML
Crystal structure of MutT/nudix family protein from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 86.394, 132.034, 64.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.260 |
| R-factor | 0.19416 |
| Rwork | 0.192 |
| R-free | 0.24086 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.082 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.062 | 0.289 |
| Number of reflections | 34806 | |
| <I/σ(I)> | 45.3 | 5.89 |
| Completeness [%] | 99.2 | 96.6 |
| Redundancy | 7 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | MgCl2, HEPES, PEG550, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
