2FM8
Crystal Structure of the Salmonella Secretion Chaperone InvB in Complex with SipA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 146.505, 146.505, 156.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.000 - 2.200 |
| R-factor | 0.19999 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.072 | 0.000 |
| Number of reflections | 66495 | |
| Completeness [%] | 99.2 | 98.7 |
| Redundancy | 8.6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 27mg/ml protein with an equilibration buffer consisting of 3.8M Na-formate, 0.9M Na-cacodylate pH 5.2 and 0.09M guanidine-hydrochloride as an additive, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
