2FM6
Zinc-beta-lactamase L1 from stenotrophomonas maltophilia (native form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979667 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 105.070, 105.070, 196.040 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.500 - 1.750 |
| R-factor | 0.1775 |
| Rwork | 0.176 |
| R-free | 0.20301 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sml |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.453 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.072 | 0.403 |
| Number of reflections | 64243 | |
| <I/σ(I)> | 7.8 | 2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | AS, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
