2FLS
Crystal structure of Human Glutaredoxin 2 complexed with glutathione
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-09 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.201, 60.201, 67.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.380 - 2.050 |
| R-factor | 0.19238 |
| Rwork | 0.190 |
| R-free | 0.23090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cq9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.502 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.380 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.115 | |
| Number of reflections | 9322 | |
| <I/σ(I)> | 13.5 | 2.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.9 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 293 | 0.1 M citrate, 25% PEG 3350, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
