2FLN
binary complex of catalytic core of human DNA polymerase iota with DNA (template A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 98.114, 98.114, 202.823 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.220 - 2.500 |
| R-factor | 0.2309 |
| Rwork | 0.226 |
| R-free | 0.27864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB CODE 2ALZ |
| RMSD bond length | 0.035 |
| RMSD bond angle | 2.961 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.058 |
| Number of reflections | 19908 |
| <I/σ(I)> | 33.5 |
| Completeness [%] | 99.1 |
| Redundancy | 10.32 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |
