2FLA
Structure of thereduced HiPIP from thermochromatium tepidum at 0.95 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.88557 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.472, 46.408, 58.988 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 0.950 |
| R-factor | 0.1213 |
| Rwork | 0.121 |
| R-free | 0.14030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 0.980 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.097 | 0.488 |
| Number of reflections | 47986 | |
| <I/σ(I)> | 6.1 | 1.4 |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.5 | 298 | ammonium sulfate, sodium citrate, NaCl, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
