2FL4
The crystal structure of the spermine/spermidine acetyltransferase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.479, 90.479, 34.598 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.200 - 1.600 |
| R-factor | 0.2135 |
| Rwork | 0.212 |
| R-free | 0.24865 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.200 | 1.642 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | 0.197 |
| Number of reflections | 19993 | |
| <I/σ(I)> | 21.3 | 4 |
| Completeness [%] | 97.5 | 78.44 |
| Redundancy | 6.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 287 | 0.1M Sodium Acetate, 3M Sodium Chloride, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
