2FL1
Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.927, 146.799, 122.885 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| Rwork | 0.203 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xa9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.540 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.135 | 0.660 |
| Number of reflections | 40878 | |
| <I/σ(I)> | 11.1 | 2.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.2 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 1.5M ammonium sulphate, 0.2M K/Na Tartrate, 0.1M Na Citrate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
