2FKZ
Reduced (All Ferrous) form of the Azotobacter vinelandii bacterioferritin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2005-07-15 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.98, 1.745, 1.739, 1.635 |
| Spacegroup name | H 3 |
| Unit cell lengths | 123.676, 123.676, 284.279 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.550 - 2.000 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.24700 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.970 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.285 |
| Number of reflections | 109019 | |
| <I/σ(I)> | 15.7 | 2.87 |
| Completeness [%] | 99.5 | 99 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Capillary batch method | 8 | 291 | 15 % ethanol, 100 mM imidazole, 200 mM magnesium chloride, pH 8.0, Capillary batch method, temperature 291K |
