2FKC
Crystal Form I of Pre-Reactive Complex of Restriction Endonuclease HinP1I with Cognate DNA and Calcium Ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-27 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97179 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 105.104, 105.104, 130.360 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.890 - 2.390 |
R-factor | 0.226 |
Rwork | 0.222 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ynm |
RMSD bond length | 0.008 |
RMSD bond angle | 1.400 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.890 | 2.480 |
High resolution limit [Å] | 2.390 | 2.390 |
Rmerge | 0.108 | 0.368 |
Number of reflections | 31659 | |
<I/σ(I)> | 19 | |
Completeness [%] | 94.7 | 97.2 |
Redundancy | 7.1 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 289 | 7.5%(v/v) ethanol, 1.5M NaCl, 100mM Bis-Tris propane pH 7.8, and 20mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | ethanol | ||
2 | 1 | 1 | NaCl | ||
3 | 1 | 1 | Bis-Tris propane | ||
4 | 1 | 1 | CaCl2 | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | NaCl | ||
7 | 1 | 2 | CaCl2 | ||
8 | 1 | 2 | H2O |