2FJK

Crystal structure of Fructose-1,6-Bisphosphate Aldolase in Thermus caldophilus

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	110
Detector technology	CCD
Collection date	2000-11-23
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9797, 0.9795, 0.9600
Spacegroup name	P 21 21 21
Unit cell lengths	98.900, 113.100, 115.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.242
Rwork	0.237
R-free	0.28600
Structure solution method	MAD
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.300
High resolution limit [Å]	2.200	2.200
Number of reflections	68172
Completeness [%]	98.0	95.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	294	18% PEG 10000, 0.1M potassium cloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K