2FJF
Structure of the G6 Fab, a phage derived VEGF binding Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 274.919, 192.229, 154.108 |
| Unit cell angles | 90.00, 117.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.650 |
| R-factor | 0.19976 |
| Rwork | 0.199 |
| R-free | 0.24835 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.213 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.060 | |
| Number of reflections | 205298 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.7 | 292 | 25% PEG 3350, 0.1 M Sodium Acetate, pH 4.7, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
