2FJ2

Crystal Structure of Viral Macrophage Inflammatory Protein-II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-01
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 21 1
Unit cell lengths	45.650, 65.770, 45.620
Unit cell angles	90.00, 91.62, 90.00

Refinement procedure

Resolution	10.000 - 2.300
R-factor	0.248
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cm9
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	SHELX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.051
Number of reflections	10149
Completeness [%]	84.9	90.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	298	100mM Sodium Citrate, 11% PEG 4000, 11% 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60
1		5.6	298	100mM Sodium Citrate, 11% PEG 4000, 11% 2-propanol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 5.60