2FIY
The crystal structure of the FdhE protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 46.261, 96.617, 140.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 96.670 - 2.100 |
| R-factor | 0.19902 |
| Rwork | 0.197 |
| R-free | 0.24264 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.407 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.424 |
| Number of reflections | 35601 | |
| <I/σ(I)> | 33.8 | 3.8 |
| Completeness [%] | 99.6 | 96.72 |
| Redundancy | 8.7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.05M (NH4)2SO4, 0.1M Tris pH8.5, 25% P 5K MME, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
