2FIT
FHIT (FRAGILE HISTIDINE TRIAD PROTEIN)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1996-05-23 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 50.600, 50.600, 268.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 25.399 * |
| Data reduction software | MADNES |
| Data scaling software | MADNES |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 1.700 | |
| Rmerge | 0.039 | 0.188 * |
| Total number of observations | 89229 * | |
| Number of reflections | 30590 | |
| Completeness [%] | 68.2 | 31 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | 1.25-1.35M AMSO4, 100MM SODIUM CACODYLATE PH6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 1 | AmSO4 | 1.25-1.35 (M) | |
| 3 | 1 | 1 | sodium cacodylate | 100 (mM) |
