2FIO
Phage phi29 transcription regulator p4-DNA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-27 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 52.400, 27.800, 192.800 |
| Unit cell angles | 90.00, 97.40, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.232 |
| Rwork | 0.229 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 13386 | |
| Completeness [%] | 0.8 | 0.59 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 20% PEG 6000, 80mM magnesium chloride, 50mM CHES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | MgCl2 | ||
| 3 | 1 | 1 | CHES | ||
| 4 | 1 | 2 | PEG 6000 | ||
| 5 | 1 | 2 | MgCl2 | ||
| 6 | 1 | 2 | CHES |
