2FIA
The crystal structure of the acetyltransferase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.679, 79.658, 83.871 |
| Unit cell angles | 90.00, 130.54, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.21228 |
| Rwork | 0.210 |
| R-free | 0.25885 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.454 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.667 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.078 | 0.493 |
| Number of reflections | 17923 | |
| <I/σ(I)> | 15.8 | 1.5 |
| Completeness [%] | 99.3 | 93.07 |
| Redundancy | 4.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 287 | 0.1M Bis-Tris, 4M Sodium Nitrate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
