2FI9
The crystal structure of an outer membrane protein from the Bartonella henselae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9798 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.527, 51.931, 62.368 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 1.800 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.23420 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.325 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.066 | 0.505 |
Number of reflections | 8970 | |
<I/σ(I)> | 21 | 1.3 |
Completeness [%] | 86.5 | 35.6 |
Redundancy | 4.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10.5 | 287 | 2M (NH4)2SO4, 0.1M CAPS pH10.5, 0.2M Li2SO4, VAPOR DIFFUSION, SITTING DROP, temperature 287K |