2FI7

Crystal Structure of PilF : Functional implication in the type 4 pilus biogenesis in Pseudomonas aeruginosa

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2004-10-13
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9796, 0.9797, 0.9719
Spacegroup name	P 21 21 2
Unit cell lengths	138.466, 68.239, 69.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.22
Rwork	0.220
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.092
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.200	2.200
Number of reflections	43694
Completeness [%]	96.1	96.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	289	100mM Na-acetate (pH 4.6), 1.6M Na-formate, 100mM DTT, 15% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K