2FI1
The crystal structure of a hydrolase from Streptococcus pneumoniae TIGR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | I 41 |
| Unit cell lengths | 71.417, 71.417, 104.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.000 - 1.400 |
| R-factor | 0.1578 |
| Rwork | 0.157 |
| R-free | 0.16992 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.171 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.046 | 0.364 |
| Number of reflections | 48700 | |
| <I/σ(I)> | 27 | 2.38 |
| Completeness [%] | 99.8 | 93.8 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 287 | 0.1M Bis0Tris, 0.05M CaCl2, 30% PEG550, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 287K |
