2FHP
Crystal Structure of Putative Methylase from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.730, 61.431, 70.579 |
| Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
| Resolution | 28.840 - 1.600 |
| R-factor | 0.18256 |
| Rwork | 0.179 |
| R-free | 0.21499 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.265 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.840 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.091 | 0.184 |
| Number of reflections | 46453 | |
| <I/σ(I)> | 15.5 | 10.8 |
| Completeness [%] | 96.3 | 76.3 |
| Redundancy | 7.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 100mM HEPES pH 7.5, 25% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
