2FH3
C-terminal half of gelsolin soaked in low calcium at pH 8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.095 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.107, 90.526, 156.584 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.070 - 2.870 |
| R-factor | 0.203 |
| Rwork | 0.200 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.093 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.940 |
| High resolution limit [Å] | 2.870 | 2.870 |
| Number of reflections | 27880 | |
| Completeness [%] | 98.4 | 85.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | micro batch under oil | 4.5 | 277 | PEG 8000, pH 4.5, micro batch under oil, temperature 277K |
