2FGY
Beta Carbonic Anhydrase from the Carboxysomal Shell of Halothiobacillus neapolitanus (CsoSCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.738, 77.636, 82.714 |
| Unit cell angles | 66.84, 78.82, 79.17 |
Refinement procedure
| Resolution | 75.380 - 2.200 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.21700 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.617 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.123 | 0.488 |
| Number of reflections | 52789 | |
| Completeness [%] | 98.0 | 94.7 |
| Redundancy | 5.7 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1M HEPES, pH 7.0, 0.2M magnesium chloride, 10% ethanol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
