2FGT

Crystal Structure of YycH from Bacillus subtilis

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.01978, 0.97958, 0.97625
Spacegroup name	P 43 21 2
Unit cell lengths	63.578, 63.578, 192.327
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.350 - 2.300
R-factor	0.255
Rwork	0.251
R-free	0.30400
Structure solution method	MAD
RMSD bond length	0.001
RMSD bond angle	1.400
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.350	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.073	0.397
Number of reflections	18254
<I/σ(I)>	19.3	4
Completeness [%]	99.4	100
Redundancy	6.8	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	0.1 M Tris_HCl, 18% PEG 3350, 100 mM calcium acetate, pH 7.0, VAPOR DIFFUSION, temperature 295K