2FGS
Crystal structure of Campylobacter jejuni YCEI protein, structural genomics
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-12-19 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 179.535, 179.535, 50.577 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.900 |
| R-factor | 0.24492 |
| Rwork | 0.243 |
| R-free | 0.27986 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y0g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.250 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.124 | 0.398 |
| Number of reflections | 11129 | |
| <I/σ(I)> | 6.2 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.1 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 3.2M AMMONIUM SULFATE, 0.1M BIS-TRIS, pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
