2FGF

THREE-DIMENSIONAL STRUCTURE OF HUMAN BASIC FIBROBLAST GROWTH FACTOR, A STRUCTURAL HOMOLOG OF INTERLEUKIN 1BETA

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	42.070, 86.610, 31.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.800 *
R-factor	0.23 *
Rwork	0.186
RMSD bond length	0.017 *
RMSD bond angle	2.900 *
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.800 *
Rmerge	0.046 *
Total number of observations	46630 *
Number of reflections	11001 *
Completeness [%]	97.4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	bFGF	30 (mg/ml)
2	1	drop	sodium succinate	200 (mM)
3	1	drop	dithiothreitol	60 (mM)
4	1	drop	ammonium sulfate	40 (%sat)
5	1	reservoir	ammonium sulfate	60 (%sat)
6	1	reservoir	sodium succinate	200 (mM)