2FG5

Crystal structure of human RAB31 in complex with a GTP analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2005-12-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.000
Spacegroup name	P 21 3
Unit cell lengths	87.769, 87.769, 87.769
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.801
Rwork	0.200
R-free	0.27380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB1YVD
RMSD bond length	0.015
RMSD bond angle	1.240
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.900
High resolution limit [Å]	2.800	6.020	2.800
Rmerge	0.058	0.021	0.745
Number of reflections	5772
Completeness [%]	99.8
Redundancy	9	8.5	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	20% isopropanol, 16% PEG 4k, 0.1M BisTris, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K