2FG1
Structure of a Protein of Unknown Function from Bacteroides thetaiotaomicron.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97928, 0.97940 |
| Spacegroup name | P 43 |
| Unit cell lengths | 62.172, 62.172, 36.505 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.250 |
| R-factor | 0.16871 |
| Rwork | 0.168 |
| R-free | 0.18127 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.259 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.282 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Number of reflections | 38567 | |
| Completeness [%] | 99.4 | 95.27 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.01M Cobalous Chloride hexahydrate, 0.1 M MES, 1.8M Ammonium Sulfate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
