2FG0

Crystal structure of a putative gamma-d-glutamyl-l-diamino acid endopeptidase (npun_r0659) from nostoc punctiforme pcc 73102 at 1.79 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0163, 0.9798
Spacegroup name	P 41 21 2
Unit cell lengths	125.080, 125.080, 97.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.480 - 1.790
Rwork	0.154
R-free	0.17200
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.480	29.480	1.860
High resolution limit [Å]	1.790	3.870	1.790
Rmerge	0.147	0.011	0.011
Number of reflections	72488	13941	11630
<I/σ(I)>	12.56	39.07	2.13
Completeness [%]	97.5		89.4
Redundancy	7.4		6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	277	1.4M Na3Citrate, 0.1M HEPES, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K, pH 7.5