2FFQ
The crystal structure of human neuronal Rab6B in its active GTPgS-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.885, 60.983, 63.685 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.780 |
| R-factor | 0.188 |
| Rwork | 0.191 |
| R-free | 0.20200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.800 | 1.880 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.052 | 0.228 |
| Number of reflections | 13846 | |
| <I/σ(I)> | 11.9 | 7.5 |
| Completeness [%] | 99.4 | 97.8 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 292.15 | 20% PEG3350, 0.2M ammonium nitrate , pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |
