2FFA
Crystal structure of ABC-ATPase H662A of the ABC-transporter HlyB in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.156, 34.770, 38.100 |
| Unit cell angles | 90.00, 98.58, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| R-factor | 0.20683 |
| Rwork | 0.205 |
| R-free | 0.24033 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mt0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.574 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 24609 | |
| <I/σ(I)> | 26 | 3.2 |
| Completeness [%] | 99.2 | 88.5 |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM Tris, 10% PEG 6000, 5% MPD, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
