2FEZ

Mycobacterium tuberculosis EmbR

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-3
Synchrotron site	ESRF
Beamline	ID14-3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-11-28
Detector	MARRESEARCH
Wavelength(s)	0.931
Spacegroup name	I 2 2 2
Unit cell lengths	78.172, 83.614, 140.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.000 - 2.000
R-factor	0.20837
Rwork	0.207
R-free	0.22842
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.061
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.400	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.089	0.470
Number of reflections	31575
<I/σ(I)>	20.6	4.2
Completeness [%]	100.0	100
Redundancy	11.1	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	0.1 M Na-Hepes, pH 7.5, 15% PEG 4000, 0.2 M magnesium choride, 5 mM dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 285K
1	VAPOR DIFFUSION, HANGING DROP	7.5	285	0.1 M Na-Hepes, pH 7.5, 15% PEG 4000, 0.2 M magnesium choride, 5 mM dithiothreitol, VAPOR DIFFUSION, HANGING DROP, temperature 285K