2FET
Synthesis of C-D-Glycopyranosyl-Hydroquinones and-Benzoquinones. Inhibition of PTP1B. Inhibition of and binding to glycogen phosphorylase in the crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2003-10-31 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.803 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 128.308, 128.308, 116.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.030 |
R-factor | 0.1881 |
Rwork | 0.188 |
R-free | 0.21300 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2prj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.250 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.090 |
High resolution limit [Å] | 2.030 | 2.030 |
Number of reflections | 56087 | |
<I/σ(I)> | 13.1 | 3.6 |
Completeness [%] | 91.7 | 88.4 |
Redundancy | 3.4 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 6.7 | 289 | 10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K |