2FE4
The crystal structure of human neuronal Rab6B in its inactive GDP-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.036, 61.125, 66.634 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.300 |
| R-factor | 0.227 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | Rab6 from Plasmodium falciparum (PDB code 1D5C) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.796 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.600 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.056 | 0.076 |
| Number of reflections | 6366 | |
| <I/σ(I)> | 10.2 | 8.8 |
| Completeness [%] | 91.6 | 91.6 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 292.15 | 0.1M sodium acetate pH 5.0, 0.15M ammonium nitrate, 20%-23% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 292.15K |
