2FDQ
crystal structure of ACBP from Armadillo Harderian Gland
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 2001-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 49.110, 49.110, 130.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.000 - 3.500 |
| R-factor | 0.228 |
| Rwork | 0.197 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1HB8 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.560 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 3901 | |
| Completeness [%] | 99.7 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 24% PEG6000, 5% NaCl, 10mM TrisHCL, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
