2FDO
Crystal Structure of the Conserved Protein of Unknown Function AF2331 from Archaeoglobus fulgidus DSM 4304 Reveals a New Type of Alpha/Beta Fold
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97902, 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.205, 48.873, 86.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.400 |
| R-factor | 0.20127 |
| Rwork | 0.199 |
| R-free | 0.25253 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 2.459 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 7822 | |
| <I/σ(I)> | 33.904 | 3.9 |
| Completeness [%] | 98.0 | 89.55 |
| Redundancy | 6.7 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 45% MPD, 0.2 M ammonium sulfate and 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
