2FCX

HIV-1 DIS kissing-loop in complex with neamine

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-01
Detector	MARRESEARCH
Wavelength(s)	0.91961
Spacegroup name	C 2 2 21
Unit cell lengths	27.005, 113.395, 95.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.420 - 2.000
R-factor	0.267
Rwork	0.267
R-free	0.29200
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.000	2.000
Number of reflections	18852
<I/σ(I)>	24.4	4
Completeness [%]	98.6	92.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	310	MPD, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K
1	VAPOR DIFFUSION, SITTING DROP	7	310	MPD, KCl, MgCl2, Na Cacodylate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 310K