2FBQ
The crystal structure of transcriptional regulator PA3006
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-17 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9793983 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 82.922, 70.141, 47.850 |
| Unit cell angles | 90.00, 111.23, 90.00 |
Refinement procedure
| Resolution | 27.570 - 1.800 |
| R-factor | 0.18549 |
| Rwork | 0.183 |
| R-free | 0.23013 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.658 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.570 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 23510 | |
| <I/σ(I)> | 36.7 | 5.4 |
| Completeness [%] | 98.7 | 87.6 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | PEG MME 5K 25%, CaCl2 0.2M, Bis-Tris 0.1M, 2mM L-Cys, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
