2FBO
Crystal Structure of the Two Tandem V-type Regions of VCBP3 (v-region-containing chitin binding protein) to 1.85 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9192 |
| Spacegroup name | P 61 |
| Unit cell lengths | 109.597, 109.597, 48.845 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.850 |
| R-factor | 0.239 |
| Rwork | 0.228 |
| R-free | 0.26900 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.06) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 5.000 | 1.850 |
| Rmerge | 0.074 | 0.040 | 0.332 |
| Number of reflections | 28179 | ||
| Completeness [%] | 97.8 | 99.9 | 84.3 |
| Redundancy | 10.9 | 14.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 1.7M Na Formate, 100 mM Na citrate, 200 mM MgCl, step soaking into 20% ethylene glycol, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
