2FBL
The crystal structure of the hypothetical protein NE1496
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-18 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97959 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 52.282, 52.282, 252.484 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.600 - 1.900 |
| R-factor | 0.22066 |
| Rwork | 0.217 |
| R-free | 0.28183 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.967 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.600 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 32884 | |
| <I/σ(I)> | 31 | 2.3 |
| Completeness [%] | 99.7 | 99 |
| Redundancy | 6.7 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | Na formate 3.5M, 0.1M Bis-Tris, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 297K, pH 7.0 |
