2FBI
The crystal structure of transcriptional regulator PA4135
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.979522 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 84.782, 84.782, 91.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.490 - 2.100 |
| R-factor | 0.22525 |
| Rwork | 0.223 |
| R-free | 0.27645 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.852 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.490 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 11144 | |
| <I/σ(I)> | 30.9 | 2.2 |
| Completeness [%] | 95.8 | 71.8 |
| Redundancy | 6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 297 | 0.2M Mg Acetate, 20% PEG3350, 0.1M Na Acetate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
