2FBE

Crystal Structure of the PRYSPRY-domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	CCD
Collection date	2005-08-14
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 41 2 2
Unit cell lengths	77.220, 77.220, 297.299
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.460 - 2.520
R-factor	0.227
Rwork	0.227
R-free	0.27700
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.800
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.610
High resolution limit [Å]	2.520	2.520
Number of reflections	30566
<I/σ(I)>	16.1	3.1
Completeness [%]	96.4	99
Redundancy	4.3	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	25% PEG 2000 MME, 0.55M KSCN, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	25% PEG 2000 MME, 0.55M KSCN, 0.1M cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K