2FB5
Structural Genomics; The crystal structure of the hypothetical membrane spanning protein from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-14 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.614, 109.609, 77.484 |
| Unit cell angles | 90.00, 90.07, 90.00 |
Refinement procedure
| Resolution | 77.620 - 1.990 |
| R-factor | 0.18325 |
| Rwork | 0.182 |
| R-free | 0.21530 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.212 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.620 | 2.070 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.090 | 0.489 |
| Number of reflections | 61484 | |
| <I/σ(I)> | 26 | 1.51 |
| Completeness [%] | 97.2 | 73.1 |
| Redundancy | 8.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 0.18M Sodium Malonate, 18% PEG3350, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
