2FAW
crystal structure of papaya glutaminyl cyclase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.953718, 1.77122 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.820, 81.200, 108.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.537 - 1.700 |
Rwork | 0.165 |
R-free | 0.20800 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 1.933 |
Data reduction software | SCALE |
Data scaling software | XDS |
Phasing software | SHARP |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.537 | 1.800 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 61500 | |
<I/σ(I)> | 41.6 | 15.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 28.9 | 29.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 85 mM Tris-HCl, 0.17 M sodium acetate, 25% PEG 4000, 15% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |