2FAV
Crystal structure of SARS macro domain in complex with ADP-ribose at 1.8 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-16 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.451, 55.553, 108.926 |
| Unit cell angles | 90.00, 91.39, 90.00 |
Refinement procedure
| Resolution | 111.800 - 1.800 |
| R-factor | 0.164 |
| Rwork | 0.162 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.511 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.800 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 41654 | |
| <I/σ(I)> | 17.7 | 7.1 |
| Completeness [%] | 99.9 | 99.97 |
| Redundancy | 10.4 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 0.1 M Imidazole, 0.9 M NaCitrate, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
