2FAU

Crystal structure of human vps26

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	98
Detector technology	CCD
Collection date	2005-02-06
Detector	MARRESEARCH
Wavelength(s)	1.072
Spacegroup name	P 21 21 21
Unit cell lengths	40.964, 76.368, 126.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.710 - 2.100
R-factor	0.235
Rwork	0.235
R-free	0.28400
Structure solution method	AB INITIO PHASING
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.710	2.160
High resolution limit [Å]	2.090	2.090
Number of reflections	22533
Completeness [%]	87.9	55.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	8% PEG 8000 100mM Na cacodylate pH 6.5 15% glycerol 10mM DTT 1mM TmCl3, VAPOR DIFFUSION, SITTING DROP, temperature 300K
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	8% PEG 8000 100mM Na cacodylate pH 6.5 15% glycerol 10mM DTT 1mM TmCl3, VAPOR DIFFUSION, SITTING DROP, temperature 300K
1	VAPOR DIFFUSION, SITTING DROP	6.5	300	8% PEG 8000 100mM Na cacodylate pH 6.5 15% glycerol 10mM DTT 1mM TmCl3, VAPOR DIFFUSION, SITTING DROP, temperature 300K