2FAE

Crystal structure of E. coli decanoyl-ACP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Wavelength(s)	0.933
Spacegroup name	P 21 21 2
Unit cell lengths	47.248, 107.497, 28.041
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.550
Rwork	0.214
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB 1L0I
RMSD bond length	0.010
RMSD bond angle	2.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.065	0.311
Number of reflections	18488
<I/σ(I)>	17	2.7
Completeness [%]	86.0	67
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	290	8-12% PEG 4000, 30 mM zinc acetate, 50 mM sodium cocadylate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K